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Spatial Gene and Protein Expression of Mouse Spleen Fresh Frozen Tissue

Stereo-CITE T FF V1.1, software version: SAW 8.1 & StereoMap 4.1
Mouse spleen Demo Data
Chip SN: C04776D6
Sample information
Kit information
Experiment information
Sequencing
Analysis result
Input file & Output and supplemental files
Data Details
Sample information
Species
Mouse (Strain: C57BL/6; Sex: male; Age: 6-8 week)
Tissue
Spleen
Embedding method
Fresh frozen
RIN value/DV
6.5
Kit information
Product
Stereo-CITE FF
Kit version
Stereo-CITE T FF V1.1
Chip size
1 cm × 1 cm
Experiment information
Section thickness
10 μm
Staining type
DAPI staining
Permeabilization time
12 min
Sequencing
Total reads
2,727,841,217
Sequencer
DNBSEQ-T7RS    
Analysis result
Mouse spleen demo data Mouse spleen demo data Mouse spleen demo data
Clustering map
  • Analyze Software Version : SAW V8.1, StereoMap V4.1
  • Bin200 Median MID : 92,454
  • Bin200 Median Gene Type : 11,526
  • Cell Bin Gene Type : 380
  • Cell Bin MID : 480
  • Sequencing Saturation : 75%
  • Fraction MID in Spots Under Tissue : 77.35%
  • Manual tissue segmentation was performed.

Input files Output and supplemental files
Input files Format Size md5sum
Sequencing FASTQ Files C04776D6_input_command_download TXT 3.68KB da8da22d1302ed3b9a6854a096956830
E250047428_L01_12_2.fq.gz FQ 48.49GB 3cd429019ad4b8229f2d812d7ca12fc1
E250047428_L01_12_1.fq.gz FQ 27.13GB ae8a4077d6fa67bbf417a92d0587c676
E250047428_L01_11_2.fq.gz FQ 46.64GB aed57f2461b4f137742cf32d3f7122d1
E250047428_L01_11_1.fq.gz FQ 26.06GB 79ea7675cebd71406f7b74145e92d6a0
E250047428_L01_10_2.fq.gz FQ 120.68GB 710d7ff0fd2c54525666717e45f49557
E250047428_L01_10_1.fq.gz FQ 55.73GB 7cdd9bf08be853b3e1f101539c29bcbe
E250047428_L01_9_2.fq.gz FQ 84.11GB bf0b848cd73fadedd338bac1b9892d8b
E250047428_L01_9_1.fq.gz FQ 38.70GB 703d32729037e500c9e0c5588662353c
C04776D6_SC_20250306_110538_4.1.1.tar.gz TAR 177.47MB 3b6cc36b4ba7145095f479a1b834c652
C04776D6.barcodeToPos.h5 H5 2.75GB 031abb7137f5692d89232f7700d0dd52
Output and supplemental files Format Size md5sum
Sequencing FASTQ Files C04776D6_output_command_download TXT 3.96KB  de6a7d07e18ff1c8c765c55335bbe21e
C04776D6_DAPI_regist.tif TIFF 147.22MB 4eed766e720430706812ba58f3c2d5d7
C04776D6.protein.adjusted.cellbin.gef GEF 413.98MB 3656ab33f4fe3950704e4a233702450d
C04776D6.protein.cellbin.gef GEF 386.69MB 6ab0f999756134f54f7a85aba7a6b4eb
C04776D6.protein.tissue.gem.gz GEM 1.44GB 830da04bf8d2bf7bad88a7b48268a32d
C04776D6.protein.tissue.gef GEF 7.86GB 71a137fd34f41959a5418f269f0377c8
C04776D6.protein.raw.gef GEF 5.26GB d414f9a53fae217faf68d98a0656b800
C04776D6.adjusted.cellbin.gef GEF 1.86MB 833d0e5aeda370d98a6ba253c867fb03
C04776D6.cellbin.gef GEF 1.28MB d940260e58f299719978467252e9c9dd
C04776D6.tissue.gem.gz GEM 794.43MB ce2a9ebe41075aa77e273e31c492aa2c
C04776D6.tissue.gef GEF 9.84GB 31062fa681ae41d2b771c4813ff5d43b
C04776D6.raw.gef GEF 2.10GB cd5320d753e2d1efaee489daf678e6a4
C04776D6.report.html HTML 70.88MB ce24c8f5d05c937f7ce25a1a323be8e9
C04776D6.visualization.tar.gz TAR 8.51GB ae315ffbb60c9b086b23854fb30d42e4
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