Transcriptomics mIF Solution

Stereo-seq mIF Product Note Stereo-seq Permeabilization Set User Manual Stereo-seq Transcritpomics Set for mIF User Manual

Solution Overview

Combining whole transcriptomics, protein detection, and tissue morphological information on the same tissue section allows for in-depth spatial multi-omics discovery. Stereo-seq v1.3 mIF solution enables spatial visualization of multiple proteins in single-cell resolution on top of the unbiased whole transcriptome information on the same tissue section. Without affecting mRNA capturing, the additional detected protein information can be integrated with gene expression data for in-depth evaluation of valuable samples, and to parse complex pathological and physiological processes.

Product Highlight

Offer flexibility in choosing antibodies based on your research needs and project design

Deliver single-cell resolution results across different species enabling more robust and translatable scientific discoveries

Detect up to 4 protein biomarkers, provide unbiased high-resolution whole transcriptomics information and assess RNA and protein spatial distributions in cells on the same fresh frozen tissue

Improved workflow and upgraded bioinformatic pipeline providing a user-friendly experience

Key features

Demo Data

Mouse brain

Tissue area: 27.95 mm²
Total reads: 1.75 G
Valid CID Read: 1,267,486,138
Median gene type count: 282
Cell count:70,035
Total transcriptome: 708.29 M
Median MID count per cell: 1,133
Median gene type count per cell: 732
Chip size: 1 cm x 1 cm

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Workflow

Tissue to Data End-to-end STOmics Solution

Perform Stereo-seq in your own laboratory with Stereo-seq Kit Solutions

Product Number	Product	Specification
211SP11118	Stereo-seq Permeabilization Set for Chip-on-a-slide V1.1	8 RXN
211KP11118	Stereo-seq Permeabilization Kit	8 RXN
210CP118	Stereo-seq Chip P Slide (1cm*1cm)	8 EA
1000033700	STOmics Accessory Kit	5 PCS
211ST13114	Stereo-seq Transcriptomics Set for Chip-on-a-slide V1.3	4 RXN
211KT13114	Stereo-seq Transcriptomics T Kit	4 RXN
210CT13114	Stereo-seq Chip T Slide (1cm*1cm)	4 EA
1000033700	STOmics Accessory Kit	5 PCS
111KL160	Stereo-seq 16 Barcode Library Preparation Kit	16 RXN
301AUX001	Stereo-seq PCR Adaptor	2 EA

FAQ

Q Can different antibodies be added in the mIF staining process? Which sources of antibodies have been tested so far?

A

The primary antibody should be mixed with different primary antibodies of different origins (primary antibody and secondary antibody should be of the same origin), and so far only primary antibodies of mouse/rabbit/rat origin have been tested.

Q When performing antibody titrations on microscopic glass slides, how many tissue sections can be mounted on one slide?

A

It is recommended to mount only one tissue section per microscopic glass slide to prevent the tissue from drying out during subsequent operations.

Q Is Stereo-seq mIF workflow applicable on any chip sizes other than 1cm x 1cm?

A

Currently not, but we are in the process of testing Stereo-seq mIF workflow on other chip sizes besides 1cm x 1cm.

Q Are there any recommended blocking solutions for non-human species?

A

We recommend to use serum and FCR blocking reagent for preparing the blocking solution. For non-human/mouse species, we recommend to substitute FcR blocking reagent with nuclease-free water.

Q Have other secondary antibodies been tested besides Alexa Fluro Plus 2nd antibodies?

A

We have only tested Alexa Fluro Plus 2nd antibodies.