Fan et al. (2023) analyzed 31 treatment-naïve CSCC samples using a combination of single-cell RNA sequencing (scRNA-seq) and Stereo-seq Transcriptomics Solution to map the heterogeneity of CSCC tumors at an unprecedented resolution. The study applied Non-negative Matrix Factorization (NMF) to scRNA-seq data to identify seven distinct transcriptional meta-programs (MP1–MP7) in CSCC cells, and these states were then spatially mapped using Stereo-seq, a high-resolution spatial transcriptomics platform develope

Tumors are highly heterogeneous tissues characterized by intricate spatial architecture, wherein cancer cells, immune cells, and stromal cells collectively constitute distinct and dynamic microenvironments across the tumor landscape (Pattabiram et al., 2025). Understanding spatial heterogeneity is critical for uncovering how tumors work and why treatments succeed or fail, but traditional transcriptomic methods cannot capture this complexity. Stereo-seq enables entire tissue sections to be studied at single-

This post outlines key principles for handling FFPE tissues in spatial transcriptomics, emphasizing RNA quality checks, proper tissue block preparation, section quality, and correct orientation. Adhering to these practices ensures reliable data, minimizing variability and enhancing downstream analysis.

To understand how the brain works, researchers need to connect molecular identity with spatial organization - from single cells to entire neural circuits. Stereo-seq enables this connection by providing single-cell resolution transcriptomics across large brain areas, allowing genes, cells, and circuits to be studied within their native spatial context.

Stay ahead of the curve with the latest developments, innovations, and resources in spatial biology from STOmics. We're pushing the boundaries of what's possible with Stereo-seq, offering nanoscale resolution across a large field of view. Dive into recent publications and exciting advancements as we continue to empower groundbreaking discoveries in neuroscience, disease research, and beyond.

Ideal for large-batch sample projects, CSRefiner currently supports three leading models in spatial omics: Cellpose, StarDist, and CellBin (more models planned). With minimal annotated local data, it fine-tunes existing models to specific image features; the fine-tuned model then segments entire/similar images, boosting efficiency while ensuring key-region accuracy.

A recent study published in Molecular Cancer [1] employs single-cell technologies to investigate tumor microenvironment (TME) heterogeneity between primary tumors and liver metastases in CRNET-LM and constructs a single-cell atlas for CRNET tumor samples. The goal is to provide new insights and research avenues for enhancing the prognosis of CRNET-LM.

This data-driven study not only reveals novel therapeutic targets for anti-tumor immunity, but also provides a translatable framework to dissect immune regulation in tumor microenvironments across cancers.

Spatial multi-omics technologies are transforming our understanding of complex biological systems with unprecedented detail. STOmics' Stereo-seq offers nanoscale resolution and an expansive field of view of up to 160 cm². In this issue, we highlight recent advances, key resources, and notable publications that showcase the power of Stereo-seq across neuroscience, disease research, and beyond.

In this blog, we explore the principles of spatial gene co-expression, evaluate the performance of computational algorithms on Stereo-seq datasets, and highlight case studies demonstrating its translational potential.