Transcriptomics Solution

Stereo-seq Chip P Slide Experiment Operational Guide Video Stereo-seq Chip T Slide Experiment Operational Guide Video Stereo-seq v1.3 Product Note

Solution Overview

STOmics Stereo-seq Transcriptomics Solution enables a “tissue-to-data” experience through in situ capturing of the whole transcriptome at nanoscale resolution and centimeter-sized field of view. We now offer the upgraded Stereo-seq Transcriptomics Solution v1.3 with refined reagent chemistry, enhanced probe design and optimized enzyme selection. You'll experience greater efficiency, broader compatibility, and a more streamlined workflow

Product Highlight

Click here to know about the upgraded Stereo-seq mIF solution!

Improved capture efficiency results in higher gene counts per cell

Currently available in 0.5cm x 0.5cm or 1cm x 1cm chip sizes.The Large Chip Designs (LCD) upgrade will be available soon

Compatible with a variety of tissue types and species as well as precious samples with low RIN values

Not limited by predetermined targets or throughput, enabling unbiased whole transcriptome discovery and the ability to combine multi-omics capabilities

Publications

Cross-species single-cell spatial transcriptomic atlases of the cerebellar cortex

Single-cell Stereo-seq Reveals Induced Progenitor Cells Involved in Axolotl Brain Regeneration

Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays

Single-cell spatial transcriptome reveals cell-type organization in the macaque cortex

Stereo-seq DEVENLOPMENTAL CELL coverpage

Spatiotemporal mapping of gene expression landscapes and developmental trajectories during zebrafish embryogenesis

Demo Data

Workflow

Tissue to Data End-to-end Solution

Advance towards a more accurate and in-depth saptial single-cell omics study with Stereo-seq's robust chemistry and efficient bioinformatics tools.

Mouse tongue single-cell clustering results (cellbin)

Mouse brain single-cell clustering results (cellbin)

Mouse ovary single-cell clustering results (cellbin)

Mouse eyeball single-cell clustering results (cellbin)

Perform Stereo-seq in your own laboratory with Stereo-seq Kit Solutions

Catalog Number	Catalog Number (US use only)	Set Name	Specifications	Version	Description
211SP11118	211SP11118-CG	Stereo-seq Permeabilization Set for Chip-on-a-slide V1.1	8 RXN	V1.1	For determining permeabilization parameters to optimize mRNA capture.
211ST13114	211ST13114-CG	Stereo-seq Transcriptomics Set for Chip-on-a-slide V1.3	4 RXN	V1.3	For generating a spatially-resolved 3' mRNA library from biological tissue sections
211ST13004	211ST13004-CG	Stereo-seq Transcriptomics Set for Chip-on-a-slide (0.5cm*0.5cm) V1.3	4 RXN	V1.3
111KL160	111KL160-CG	Stereo-seq 16 Barcode Library Preparation Kit	16 RXN	V1.0	Designed for library preparation of samples using Stereo-seq technology, enables the addition of sample barcodes and library construction.
301AUX001	301AUX001-CG	Stereo-seq PCR Adaptor	2 EA	/	Compatible with PCR thermal cycler as a heating unit.

FAQ

Q Can I use used chips to practice tissue section placement?

It can be used for practice.

Q Can samples of different tissue types be analyzed on a same STOmics Gene Expression Chip?

No, samples of different tissue types need to be tested for different permeabilization conditions. In addition, different samples should not be processed on the same chip to prevent cross-contamination.

Q Which sequencing platform is applicable to Stereo-seq Transcriptomics Set library?

STOmics Gene Expression Set-S1 library can be sequenced on DNBSEQ-G400RS, MGISEQ-2000RS and DNBSEQ-T7RS platforms.

Q Can the library of Stereo-seq Transcriptomics Set be pooled with other libraries for sequencing?

No, Stereo-seq Transcriptomics Set library requires different sequencing protocols and sequencing reagents compared to other libraries.

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