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Stereo-seq Transcriptomics Solution
Designed With Nanoscale Resolution to Address In Depth Spatial Biology Questions
Stereo-seq Transcriptomics Solution
Stereo-seq Transcriptomics Solution
Designed With Nanoscale Resolution to Address In Depth Spatial Biology Questions
Stereo-seq Transcriptomics Solution
Designed With Nanoscale Resolution to Address In Depth Spatial Biology Questions
Solution Overview Product Highlight Demo Data Workflow Tissue to Data End-to-end Solution Stereo-seq Kit Solutions FAQ
Solution Overview
STOmics Stereo-seq Transcriptomics Set for Chip-on-a-slide enables a “tissue-to-data” solution through in situ capture of the whole transcriptome, at nanoscale resolution and centimeter-sized field of view. Stereo-seq Transcriptomics Solution utilizes DNB patterned array chips loaded with spatially-barcoded probes that capture and prime poly-adenylated mRNA from tissue sections in situ. Each cDNA synthesized from mRNA captured on a particular spot is linked to its spatially-barcoded probe, allowing subsequent gene expression mapping of a tissue section following sequencing and visualization analysis using the StereoMap visualization platform.
Solution Overview
Product Highlight
Unbiased in situ capturing of the whole transcriptome with true nanoscale resolution
Proprietary cell segmentation algorithm integrated on Stereo-seq Analysis Workflow (SAW) transforming data to information
Unlimited by species and tissue types, also compatible with both fresh frozen and PFA fixed samples for a more extensible application
Larger capture area with chip sizes up to 1cm by 1cm without compromising the capture efficiency
Demo Data
Adult mouse brain
  • Section size: ~10.5 mm²
  • Number of mapped spatial barcodes (CIDs) under tissue: ~15.3 M
  • Number of genes: 578 (bin 14, 14 × 14 DNB, equivalent to ~1 medium size cell)
Workflow
Tissue to Data End-to-end Solution Tissue to Data End-to-end Solution Tissue to Data End-to-end Solution
Tissue to Data End-to-end Solution
Advance towards a more accurate and in-depth saptial single-cell omics study with Stereo-seq's robust chemistry and efficient bioinformatics tools.


Perform Stereo-seq in your own laboratory
with Stereo-seq Kit Solutions
Tissue to Data End-to-end Solution Tissue to Data End-to-end Solution Tissue to Data End-to-end Solution
FAQ
Q Can I use used chips to practice tissue section placement?
A

It can be used for practice.

Q Can samples of different tissue types be analyzed on a same STOmics Gene Expression Chip?
A

No, samples of different tissue types need to be tested for different permeabilization conditions. In addition, different samples should not be processed on the same chip to prevent cross-contamination.

Q Which sequencing platform is applicable to Stereo-seq Transcriptomics Set library?
A

STOmics Gene Expression Set-S1 library can be sequenced on DNBSEQ-G400RS, MGISEQ-2000RS and DNBSEQ-T7RS platforms.

Q Can the library of Stereo-seq Transcriptomics Set be pooled with other libraries for sequencing?
A

No, Stereo-seq Transcriptomics Set library requires different sequencing protocols and sequencing reagents compared to other libraries.

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