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Spatial Gene Expression of Mouse Tongue Fresh Frozen Tissue

Stereo-seq T FF V1.3, software version: SAW 8.1 & StereoMap 4.1
Mouse tongue Demo Data
Chip SN: B04372C214
Sample information
Kit information
Experiment information
Sequencing
Analysis result
Input file & Output and supplemental files
Data Details
Sample information
Species
Mouse (Strain: C57BL/6; Sex: Male; Age: 6-8 week)
Tissue
Tongue
Embedding method
Fresh frozen
RIN value/DV
4.9
Kit information
Product
Stereo-seq FF
Kit version
Stereo-seq T FF V1.3
Chip size
0.5 cm × 0.5 cm
Experiment information
Section thickness
10 μm
Staining type
H&E staining
Permeabilization time
12 min
Sequencing
Total reads
1,184,524,188
Sequencer
DNBSEQ-G400RS
Analysis result
Clustering map
  • Analyze Software Version : SAW V8.1, StereoMap V4.1
  • Bin200 Median MID : 113,966
  • Bin200 Median Gene Type : 11,790
  • Cell Bin Gene Type : 916
  • Cell Bin MID : 1,698
  • Sequencing Saturation : 73.50%
  • Fraction MID in Spots Under Tissue : 81.46%

Input files Output and supplemental files
Input files Format Size md5sum
Sequencing FASTQ Files B04372C214_input_command_download TXT 3.5K df0bfe6c3448e892d6aaa87ee9abec98
V350285673_L04_read_2.fq.gz FQ 9.92GB b15557d2e72f2ef6e5bbc1a3b7fc8aa4
V350285673_L04_read_1.fq.gz FQ 8.62GB 4f1c6404954aff29fd28c7c143a78848
V350285673_L03_read_2.fq.gz FQ 9.80GB 0120b8cfe431ca829e4f3a53cfbbb063
V350285673_L03_read_1.fq.gz FQ 8.55GB 69bd470f7fe8f160b28553dfb74e3ea1
V350285673_L02_read_2.fq.gz FQ 8.64GB e535bfa9b2e0b409d6861425f87d13ae
V350285673_L02_read_1.fq.gz FQ 7.52GB 7e0b45a1e7c75eab657518c3f2d8e45c
V350285673_L01_read_2.fq.gz FQ 9.63GB c0eb296b85e22dc4cac5e7a75f6285ab
V350285673_L01_read_1.fq.gz FQ 8.43GB 52995eb3764ef3fd4bd449927fad1723
B04372C214_SC_20240925_145336_4.1.0.tar.gz TAR 418.55MB f83e7d6a52c1ff2cbbf1f23a69362bd6
B04372C214.barcodeToPos.h5 H5 808.98MB 8db1a79f230e8f9fe131bc3ce646ad9a
Output and supplemental files Format Size md5sum
Sequencing FASTQ Files B04372C214_output_command_download TXT 2.87KB dd2ef14cede6a1f992057d876e1faccd
B04372C214_HE_regist.tif TIFF 278.80MB a5cfcadc976a5ac71bcc8bfb4d89d5a5
B04372C214.adjusted.cellbin.gef GEF 583.47MB ea26b48e6d31f5bbadba2cc58e7b558f
B04372C214.cellbin.gef GEF 348.92MB 0305adddaa00caaf232bc8fc77d6854e
B04372C214.tissue.gem.gz GEM 343.85MB 946fe2142bd6b43f10186f3e57752b7c
B04372C214.tissue.gef GEF 3.77GB e382775a41fe2453f3bd050ef2871953
B04372C214.raw.gef GEF 879.11MB 6156ba1530888cbbb89948913f050d4e
B04372C214.report.tar.gz TAR (HTML) 12.57MB d378f5e9c6cb1f242987796549de6f9e
B04372C214.visualization.tar.gz TAR 2.74GB 24c18f651e601f721c2733090552be78
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