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Spatial Gene Expression of Mouse Brain Fresh Frozen Tissue

Stereo-seq T FF V1.3, software version: SAW 8.1 & StereoMap 4.1
Mouse brain Demo Data
Chip SN: C04042E3
Sample information
Kit information
Experiment information
Sequencing
Analysis result
Input file & Output and supplemental files
Data Details
Sample information
Species
Mouse (Strain: C57BL/6; Sex: Male; Age: 8-9 week)
Tissue
Whole brain
Embedding method
Fresh frozen
RIN value/DV
7
Kit information
Product
Stereo-seq FF
Kit version
Stereo-seq T FF V1.3
Chip size
1 cm × 1 cm
Experiment information
Section thickness
10 μm
Staining type
H&E staining
Permeabilization time
12 min
Sequencing
Total reads
1,577,388,478
Sequencer
DNBSEQ-G400RS
Analysis result
Clustering map
  • Analyze Software Version : SAW V8.1, StereoMap V4.1
  • Bin200 Median MID : 60,353
  • Bin200 Median Gene Type : 9,059
  • Cell Bin Gene Type : 735
  • Cell Bin MID : 1,223
  • Sequencing Saturation : 46.20%
  • Fraction MID in Spots Under Tissue : 92.16%

Input files Output and supplemental files
Input files Format Size md5sum
Sequencing FASTQ Files C04042E3_input_command_download TXT 4.9K 55a61f36656e23e69f4c046cd4f70e10
V350282428_L04_read_2.fq.gz FQ 9.90GB 94befe7c81ae48e6512c76fca9067173
V350282428_L04_read_1.fq.gz FQ 8.89GB 0de30ac4bbd8dd0f2cee11978f7f97ed
V350282428_L03_read_2.fq.gz FQ 9.90GB 3143732db7622bae4f6aba674b832dfb
V350282428_L03_read_1.fq.gz FQ 8.87GB 2847a0216376404c53325676c5f293f8
V350282428_L02_read_2.fq.gz FQ 9.95GB 6fd0959a0bc97c7a3d3df2e40a7d3fae
V350282428_L02_read_1.fq.gz FQ 9.06GB 7a1485131d05d5c55de0d8b24ae60675
V350282428_L01_read_2.fq.gz FQ 10.03GB bfe2aced6a48e17aa4628939fc8d4630
V350282428_L01_read_1.fq.gz FQ 9.07GB 087d900f93dd718e0b61e9c4b434c74d
V350272809_L01_read_2.fq.gz FQ 12.99GB 5d240fef169381c28812fd16a01e2bd5
V350272809_L01_read_1.fq.gz FQ 10.18GB 5b0f166e29adc27d967f6523a1ab88c0
V350264830_L01_read_2.fq.gz FQ 4.10GB 6869fe802cba3137cf301605edc2a7f2
V350264830_L01_read_1.fq.gz FQ 4.28GB c45b2e1bf606410b0e03aea90c400ebe
C04042E3_SC_20240925_142342_4.1.0.tar.gz TAR 1.09GB ab7b57c94d5a14845a41918b69f685d0
C04042E3.barcodeToPos.h5 H5 3.02GB 5f28fad742bbc9cdb68e26be7b66fe2e
Output and supplemental files Format Size md5sum
Sequencing FASTQ Files C04042E3_output_command_download TXT 2.84KB f0fdfeea11c6091485f9c9719cb4d4c1
C04042E3_HE_regist.tif TIFF 969.92MB 0ab95b22bc412901feb64fd901482c10
C04042E3.cellbin.gef GEF 737.70MB 451c6fb8763a049a189d81f3fe548dee
C04042E3.adjusted.cellbin.gef GEF 1.66GB e368aafc3fd14398deac6bf587cd8e86
C04042E3.tissue.gem.gz GEM 1.20GB 73f548f82dc1ca72898f48f9511842b8
C04042E3.tissue.gef GEF 15.24GB 91b445ad833c43ce85b1f4d46a75c97d
C04042E3.raw.gef GEF 2.52GB 360067888ef7fe35f52dc0b74a3127e6
C04042E3.report.tar.gz TAR (HTML) 45.62MB 0485143f4433bd06c3a5100795759053
C04042E3.visualization.tar.gz TAR 10.41GB 26acdc77791989745e90ec4e5cba37f5
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