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Spatial Gene Expression of Mouse eyeball Fresh Frozen Tissue

Stereo-seq T FF V1.3, software version: SAW 8.1 & StereoMap 4.1
Mouse eyeball Demo Data
Chip SN: C04687E314
Sample information
Kit information
Experiment information
Sequencing
Analysis result
Input file & Output and supplemental files
Data Details
Sample information
Species
Mouse  (Strain: C57BL/6; Sex: Male; Age: 6-8 week)
Tissue
Eyeball
Embedding method
Fresh frozen
RIN value/DV
8.04
Kit information
Product
Stereo-seq FF
Kit version
Stereo-seq T FF V1.3
Chip size
0.5 cm × 0.5 cm    
Experiment information
Section thickness
10 μm
Staining type
H&E staining
Permeabilization time
12 min
Sequencing
Total reads
662,311,476
Sequencer
DNBSEQ-G400RS
Analysis result
Clustering map
  • Analyze Software Version : SAW V8.1, StereoMap V4.1
  • Bin200 Median MID : 45,680
  • Bin200 Median Gene Type : 7,545
  • Cell Bin Gene Type : 586
  • Cell Bin MID : 1,035
  • Sequencing Saturation : 86.30%
  • Fraction MID in Spots Under Tissue : 56.70%

Input files Output and supplemental files
Input files Format Size md5sum
Sequencing FASTQ Files C04687E314_input_command_download TXT 2.19KB 36b7467c70bce64ae0b6baf8aa786c2d
V350284656_L04_read_2.fq.gz FQ 10.03GB 2372ac1da8298c5e430ebd3ce4223cee
V350284656_L04_read_1.fq.gz FQ 9.25GB 01a9ee866bffae5336c69c0b5e398428
V350284656_L03_read_2.fq.gz FQ 10.37GB 9f1e72c446f1c891f4c062cfd6c1ade4
V350284656_L03_read_1.fq.gz FQ 10.03GB b997451d4c04536f7921c9401d3aec14
C04687E314_SC_20240926_173246_4.1.0.tar.gz TAR 270.52MB ae9ddc3a77181ae565f1effd972d65e2
C04687E314.barcodeToPos.h5 H5 1.00GB 29c0218f6b12c503c798e252a6c314c1
Output and supplemental files Format Size md5sum
Sequencing FASTQ Files C04687E314_output_command_download TXT 2.87KB 3bda47c6b2a8ca8a208ff10bd4244b9b
C04687E314_HE_regist.tif TIFF 223.79MB 3cab17d71f95201e65048612e4831553
C04687E314.cellbin.gef GEF 55.81MB 182c610976f448ce25df6bb2dc73d0ec
C04687E314.adjusted.cellbin.gef GEF 90.58MB 3e5be5d05a7da66d2193029409298228
C04687E314.tissue.gem.gz GEM 70.57MB 7a2fb1a507cdd0896d2f196fb7271f9b
C04687E314.tissue.gef GEF 1.39GB bea37d68aaf25204259a31aa4ea09340
C04687E314.raw.gef GEF 245.93MB 40ecbb3d6703333ebb56ebf804459ada
C04687E314.report.tar.gz TAR (HTML) 9.88MB 9988347d9b7dfe425bea906080bf7f74
C04687E314.visualization.tar.gz TAR 840.72MB bcb1b03a896b622e6712f81868fd0355
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