Mouse thymus A02677B5

Spatial Gene and Protein Expression of Mouse thymus Fixed Frozen Tissue

Stereo-CITE T FF V1.0, software version: SAW 8.1 & StereoMap 4.1

Stereo-CITE Solution

Mouse Thymus Demo Data

Chip SN: A02677B5

Sample information

Kit information

Experiment information

Sequencing

Analysis result

Input file & Output and supplemental files

Data Details

Sample information

Species :

Mouse (Strain: C57BL/6; Sex: Male; Age: 6-8 week)

Tissue :

Thymus

Embedding method :

Fixed frozen

RIN value :

8.5

Kit information

Product :

Stereo-seq FF

Kit version :

Stereo-CITE T FF V1.0

Chip size :

1 cm × 1 cm

Experiment information

Section thickness :

10 μm

Staining type :

DAPI staining

Permeabilization time :

14 min

Sequencing

Total reads :

1,346,328,616 (RNA)

1,307,126,096 (Protein)

Sequencer :

DNBSEQ-T7RS, DNBSEQ-G400RS

Analysis result

Clustering map

Analyze Software Version : SAW V8.1, StereoMap V4.1
Bin200 Median MID : 122,252(RNA), 117,648(Protein)
Bin200 Median Gene Type : 10,158
Bin20 Median MID : 1,267(RNA), 1,220(Protein)
Bin20 Median Gene Type : 585
Cell Bin Gene Type : 277
Cell Bin MID : 543(RNA), 540(Protein)
Sequencing Saturation : 53.10%
Fraction MID in Spots Under Tissue : 72.06%
Manual tissue segmentation was performed.

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Input files Output and supplemental files

Input files	Format	Size	md5sum
A02677B5_input_command_download	TXT	4.31KB	f7471a89b5132c158f16b24cbde14ca8
V350248064_L04_read_1.fq.gz	FQ	12.59GB	38c13990ff07d30153a99985a108641c
V350248064_L04_read_2.fq.gz	FQ	16.45GB	e8d85e6657ce7e5b2f666ccc69f9cfd9
V350248064_L03_read_1.fq.gz	FQ	12.35GB	be0ddf46a81988721f76b5fa039ba981
V350248064_L03_read_2.fq.gz	FQ	16.09GB	c7f014dbd2138fc3c94c8428d8c8480f
V350248064_L02_read_1.fq.gz	FQ	12.45GB	17c492a8e6044225b52d8476cc7ee6fa
V350248064_L02_read_2.fq.gz	FQ	16.29GB	3428b2a38ef27a188285fc0a3a7ef2d5
V350248064_L01_read_1.fq.gz	FQ	11.99GB	be1d284d359928bbc91dae473c410273
V350248064_L01_read_2.fq.gz	FQ	15.69GB	2ccd4358f9f9557538d668a5c70754f3
E150023160_L01_11_1.fq.gz	FQ	19.22GB	580351a66c617a1999b4fb4d6d2fff21
E150023160_L01_11_2.fq.gz	FQ	39.43GB	f05b16c596e20535cfad8016b6904a4e
E100079141_L01_4_1.fq.gz	FQ	24.26GB	1607859a0ffb6b3111612916f467c5ca
E100079141_L01_4_2.fq.gz	FQ	47.90GB	1039333978cd99974c2f623f3c3d8676
A02677B5_SC_20241119_211910_4.1.0.tar.gz	TAR	603.76MB	be8ff3efecb421709b665a2e11bf7c77
A02677B5.barcodeToPos.h5	H5	3.73GB	1146c50b69590e58d973992df6c28c05

Output and supplemental files	Format	Size	md5sum
A02677B5_output_command_download	TXT	4.46KB	9c5a80f6d6b6effae8fc4db37fdd1406
A02677B5_DAPI_regist.tif	TIFF	446.33MB	deda64545d448f469cc555989af37b58
A02677B5.protein.adjusted.cellbin.gef	GEF	227.27MB	8c18048a8893bc4fd76600e867785113
A02677B5.protein.cellbin.gef	GEF	201.65MB	ad2b6bda66199a8381bf07b3c14a95b8
A02677B5.protein.tissue.gem.gz	GEM	375.85MB	3aa14daa1f613235529fd57e43a82759
A02677B5.protein.tissue.gef	GEF	4.65GB	6607b471eac3ffcb24dedb3c0facd4b3
A02677B5.protein.raw.gef	GEF	1.57GB	6fbf16388e89d36c4b8eedd998b0c284
A02677B5.adjusted.cellbin.gef	GEF	1.00GB	da735a8fee02998429eb93ae8737a117
A02677B5.cellbin.gef	GEF	715.81MB	7df7677f492947bb53907425af350989
A02677B5.tissue.gem.gz	GEM	518.18MB	6113d389a9e6c0c2f4d26968a45733a9
A02677B5.tissue.gef	GEF	7.67GB	50caffedd693fea95da0566643f8ab85
A02677B5.raw.gef	GEF	1.57GB	8fbe06f6df22da7908264c0d3d52cf10
A02677B5.report.tar.gz	TAR	33.03MB	7c107a6d2f25f25cf0da756307bbc4c4
A02677B5.visualization.tar.gz	TAR	5.07GB	acce1c90b37de0c9537b80cc3343a192

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