Mouse brain SS200000135TL_D1

Spatial Gene Expression of Mouse Brain Fresh Frozen Tissue

Stereo-seq T FF V1.2, software version: SAW 5.5 & StereoMap 1.0

Stereo-seq Transcriptomics Solution

Mouse Brain Demo Data

Chip SN: SS200000135TL_D1

Sample information

Kit information

Experiment information

Sequencing

Analysis result

Input file & Output and supplemental files

Data Details

Sample information

Species :

Mouse (Strain: C57BL/6; Sex: Male; Age: 6-8 week)

Tissue :

Semi brain

Embedding method :

Fresh frozen

RIN value :

Kit information

Product :

Stereo-seq FF

Kit version :

Stereo-seq T FF V1.2

Chip size :

1 cm × 1 cm

Experiment information

Section thickness :

10 μm

Staining type :

ssDNA staining

Permeabilization time :

12 min

Sequencing

Total reads :

1,002,214,171

Sequencer :

DNBSEQ-T7RS

Analysis result

Clustering map

Analyze Software Version : SAW V5.5, StereoMap V1.0
Bin200 Median MID : 35,757
Bin200 Median Gene Type : 6,726
Cell Bin Gene Type : 196
Cell Bin MID : 335
Sequencing Saturation : 79.70%
Fraction MID in Spots Under Tissue : 83.06%
The cellbin result of this sample was manually adjusted and annotated.

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Input files Output and supplemental files

Input files	Format	Size	md5sum
SS200000135TL_D1_input_command_download	TXT	1.88KB	3c2ddf1d9022ae367fb65fc59cf842bc
E100026571_L01_trim_read_1.fq.gz	FQ	32.57GB	1dbebc7d5fb286b5537aea99108debca
E100026571_L01_trim_read_2.fq.gz	FQ	79.23GB	21b32ff0faaedc094dbdc47174c75d97
SS200000135TL_D1_20220527_201353_1.1.0.tar.gz	TAR	166.63MB	8512b901ba8f68ada845fc517ecb7f3a
SS200000135TL_D1_20220527_201353_1.1.0.ipr	IPR (H5)	99.00KB	4dc5091474336b9ca984b86c84521abc
SS200000135TL_D1.barcodeToPos.h5	H5	4.42GB	e5a9b6535cb6a8616e01fc4105bf6026

Output and supplemental files	Format	Size	md5sum
SS200000135TL_D1_output_command_download	TXT	2.52KB	8ddc1ed78a87fd99a4f396ed558fc117
SS200000135TL_D1.RPI	RPI(H5)	525.09MB	c897e267c356b82ec511b4f4c967cd41
SS200000135TL_D1.cellbin.gef	GEF	204.61MB	331919d23c9f7fa9f65f5cc072ecf410
SS200000135TL_D1.tissue.gem.gz	GEM	355.93MB	2015f9228ff0a9ebb2a8315696536de9
SS200000135TL_D1.tissue.gef	GEF	600.37MB	5cf599db3b0a1f3e2ea4074b8d9cdbf3
SS200000135TL_D1.report.html	HTML	13.22MB	1aefa843b301a8d55033ff444085bb8a
SS200000135TL_D1.gef	GEF	7.48GB	3a27339733b5114737dae4ccb196c09b
SS200000135TL_D1_regist.tif	TIFF	667.65MB	129dcd4c8ddf03c64f57c13ec50fa255

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