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Spatial Gene Expression of Rat Brain Fresh Frozen Tissue

Stereo-seq T FF V1.2, software version: SAW 7.0 & StereoMap 3.0
Rat Brain Demo Data
Chip SN: B01207E3G4
Sample information
Kit information
Experiment information
Sequencing
Analysis result
Input file & Output and supplemental files
Data Details
Sample information
Species
Rat (Strain: SD; Sex: Male; Age: 6-8 week)
Tissue
Brain
Embedding method
Fresh frozen
RIN value : 
7
Kit information
Product
Stereo-seq FF
Kit version
Stereo-seq T FF V1.2
Chip size
2 cm × 3 cm
Experiment information
Section thickness
10 μm
Staining type
DAPI staining    
Permeabilization time
12 min
Sequencing
Total reads
28,020,201,071
Sequencer
DNBSEQ-T20
Analysis result
Clustering map
  • Analyze Software Version : SAW V7.0, StereoMap V3.0
  • Bin200 Median MID : 68,103
  • Bin200 Median Gene Type : 8,217
  • Cell Bin Gene Type : 514
  • Cell Bin MID : 1,031
  • Sequencing Saturation : 93.60%
  • Fraction MID in Spots Under Tissue : 84.60%

Input files Output and supplemental files
Input files Format Size md5sum
B01207E3G4_input_command_download TXT 45.5KB cdd32db2083ebbd2fac47b4574414be5
Output and supplemental files Format Size md5sum
B01207E3G4_output_command_download TXT 2.77KB ed65aeedf6764844eef4976435425e17
B01207E3G4.rpi RPI(H5) 3.32GB 04855edb6a1006275aa00ed004bd1ba9
DAPI_B01207E3G4_regist.tif TIFF 5.07GB fa1987e2c3c1d494ccb247caa429d74a
B01207E3G4.adjusted.cellbin.gef GEF 3.42GB 650d2bf7e2448497309e9ce59067816a
B01207E3G4.cellbin.gef GEF 1.64GB 3f7174aee2f371743caeb14a84a0cddd
B01207E3G4.tissue.gem.gz GEM 2.04GB e47ac4e81d8ade7586419ac7306ee06b
B01207E3G4.tissue.gef GEF 4.19GB 5dc51dfd4928f2c017206c43830f1d1f
B01207E3G4.gef GEF 39.13GB 2083ca94e6a424f5b273b911412b22da
B01207E3G4.report.html HTML 47.44MB c96c1581577543505c12edca9a37ec64
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