Paper Review

Recently, a study published in Neuron, titled “Spatiotemporal transcriptomic maps of mouse intracerebral hemorrhage at single-cell resolution” generated a single-cell resolution transcriptomic maps of mouse intracerebral hemorrhage (ICH) across nine time points (3 hours to 28 days) using Stereo-seq - high resolution spatial whole transcriptome profiling technology. 

Intracerebral hemorrhage (ICH), a devastating form of stroke, contributes significantly to global morbidity and mortality. However, due to its complex pathological mechanisms and the lack of precise therapeutic targets, no specific treatments are currently available.

This study employs Stereo-seq to map the spatiotemporal changes in gene expression following ICH, identifying 26 main cell types and 100 subclasses, revealing distinct spatial and temporal gene expression patterns. Among these 100 cell subclasses, 17 were found to critically influence the pathological mechanisms of ICH. Significant findings include increased brain water content in the ipsilateral hemisphere three days post-ICH, substantial lesion volume (8.77 ± 1.68 mm³), and a notable decrease in normal myelin area around the lesion compared to naive mice. Furthermore, astrocytes displayed dynamic molecular responses over time, secreting wound-healing-associated factors initially and expressing genes related to lipid metabolism in later stages. Immediate-early genes were activated in the cortex beyond day seven, highlighting the temporal progression of cellular responses.

The research provides novel insights into the pathophysiological processes triggered by ICH, emphasizing the importance of continued investigation in this area. This data is now available at https://db.cngb.org/stomics/stmich/ for interactive exploration, serving as an invaluable tool for advancing our understanding of ICH.

Find out more about the research: https://www.cell.com/neuron/abstract/S0896-6273(25)00309-5

About STOmics Stereo-seq:

STOmics offers the most advanced spatiotemporal multi-omics technology, enabling unbiased discovery to answer biological questions in scientific research and clinical applications. Currently, we offer spatial transcriptomics solutions, including Stereo-seq v1.3 for fresh frozen samples, Stereo-seq OMNI for FFPE samples, Stereo-seq Large Chip Designs (LCD) for centimeter-level fresh frozen samples (now up to 2cm x 3cm), and a spatial multi-omics solution - Stereo-CITE for high-plex spatial proteo-transcriptome co-detection.

https://en.stomics.tech/ 

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