Stereo-seq Transcriptomics Set library can be sequenced on DNBSEQ-G400RS, MGISEQ-2000RS and DNBSEQ-T7RS platforms.
No, samples of different tissue types need to be tested for different permeabilization conditions. In addition, different samples should not be processed on the same chip to prevent cross-contamination.
It can be used for practice.
Yes, they are different.
It is recommended to determine whether it needs to be repeated according to the purpose of the experiment.
The workflow of Stereo-seq Permeabilization Set takes around 4h; the workflow of
Please refer to the kit label.
The set is applicable to all common animal samples.
The tissue size should be no more than 0.9 cm x 0.9 cm x 2cm, and appropriate cryomold or stainless- steel based mold should be chosen based on the tissue size. To avoid RNA degradation, tissue embedding should be finished within 30 min after tissue harvest. Excess liquid on the tissue should be removed to avoid ice formation during embedding. Air bubbles should be avoided when filling the OCT in the Cryomold or stainless-steel.
The distortion of tissue could be caused by tissue compression during tissue harvest. In this case, tissue harvest method should be adjusted accordingly. The volume expansion of the OCT during freezing could also lead to tissue distortion. The embedding conditions should be adjusted according to the specific situation, such as, considering changing Cryomold or stainless-steel based mold size or material.